Table 1 Bacterial strains and plasmids used in this study
Strain or plasmid | Description | Source or reference(s) |
|---|---|---|
S. enterica serovar Typhimurium | ||
SV5015 | SL1344 his+ | J. Casades’s |
MH-C1 | SV5015 hilA::Cm | [20] |
MHS-1 | SV5015 ΔhilA | [20] |
SV5258 | SV5015 ΦinvF-lacZY | J. Casades’s |
SV5258HilA | SV5258 ΔhilA::Cm | This work |
MHS-2 | SV5015 sipA::3XFLAG | This work |
MHS-3 | MHS-1 sipA::3XFLAG | This work |
LB5000 | rLT− rSA− rSB− | [21] |
Escherichia coli | ||
E. coli O157:H7 | does not produce either Shiga-like toxin I or II (ATCC N° 43888) | ATCC |
AAG1 | MG1655 ΔLacZYA | [22] |
AAG1H | AAG1 Δhns | This work |
AAG1IHFA | AAG1 ΔihfA::Cm | This work |
AAG1IHFB | AAG1 ΔihfB::Cm− | This work |
AAG1HA | AAG1 Δhns ΔihfA::Cm | This work |
AAG1HB | AAG1 Δhns ΔihfB::Cm | This work |
AAG1ΔRSygeH | AAG1 ΔRSygeH integrated attB Kmr | This work |
AAG1HΔRSygeH | AAG1H + λRSygeH | This work |
AAG1HAλRSygeH | AAG1HA + λRSygeH | This work |
AAG1HBλRSygeH | AAG1HB + λRSygeH | This work |
EAEC 042 | Cmr Smr Tcr | I. Henderson |
042HNS | EAEC 042 hns | This work |
042YgeH | EAEC 042 ygeH | This work |
042YgeH3X | EAEC 042 ygeH::3xFLAG | This work |
042YgeH3XHNS | 042YgeH3X Δhns | This work |
Plasmids | ||
pKD4 | bla FRT ahp FRT PS1 PS2 oriR6K Kmr Cbr | [23] |
pKD3 | bla FRT cat FRT PS1 PS2 oriR6K Cmr Cbr | [23] |
pKD46 | bla PBAD gam bet exo pSC101 oriTS Cbr | [23] |
pCP20 | bla cat cI 857 l PR flp pSC101 oriTS Cbr | [24] |
pSUB11 | 3xFLAG- and Kmr-coding template vector | [25] |
pBAD18 | reppMB1 p araBAD Cbr | [26] |
pBADHilA | pBAD18 + hilA SV5015 | This work |
pBADEilA | pBAD18 + eilA EAEC042 | This work |
pBADYgeH042 | pBAD18 + ygeH EAEC042 | This work |
pBADYgeHO157 | pBAD18 + ygeH O157:H7 | This work |
pRS551 | Promoterless vector lacZ Kmr Cbr | [27] |
pYgeH042 | ygeH 042 promoter cloned into pRS551 | This work |