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Figure 2 | BMC Microbiology

Figure 2

From: HilA-like regulators in Escherichia coli pathotypes: the YgeH protein from the enteroaggregative strain 042

Figure 2

The HilA-like proteins EilA and YgeH from EAEC 042 are both able to complement in trans the ΔHilA phenotype in S. Typhimurium. Panel A and B: SDS-PAGE 10% from whole secreted protein from strains MHS-2 (SV5015 sipA::3xFLAG) and MHS-3 (sipA::3xFLAG hilA) stained with Coomassie brilliant blue. C and D: Western blots from whole secreted protein from strains MHS-2 and MHS-3. A and C: Samples obtained from cultures grown in LB medium. B and D: Samples obtained from cultures grown in LB 0.2% L-arabinose. The SipA::3xFLAG protein was detected using monoclonal anti-flag antibody. The asterisks indicate the expression of other effector proteins according to [36], which are also activated by HilA-like proteins.

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BMC Microbiology

ISSN: 1471-2180

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