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Figure 2 | BMC Microbiology

Figure 2

From: Genetic and structural identification of an O-acyltransferase gene (oacC) responsible for the 3/4-O-acetylation on rhamnose III in Shigella flexneri serotype 6

Figure 2

SDS-PAGE and Western blot of LPS from wild-type strains 51579 (serotype 6) and Sf301 (serotype 2a), their oacC and oacB deletion mutants, and complementation transformants by plasmids pSQZ4 and pSQZ5. A. Sliver-staining detection of LPS profiles on 15% polyacrylamide gels. B. The LPS separated by SDS-PAGE were transferred onto a PVDF membrane and hybridized with anti-O-factor 9 serum. An anti-rabbit antibody labeled with fluorescent IRDye 800 (Rockland) was used as the secondary antibody. Fluorescence was detected using Odyssey Infrared Imaging System (LI-COR).

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BMC Microbiology

ISSN: 1471-2180

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