Table 3 Primer sequences
Primer name | Sequence 5’-3’ | Target |
|---|---|---|
comE1 | cgagctc ggaagaattggttattgcgcgtg | orf2[10] forward - Sac I |
comE2 | cgggatcc cgggggatgacctgttgcttg | orf2[10] reverse Bam HI |
comE3 | tcccccggg ggagtcgtgtgtattcgacagcgg | P-orf2-folC[10] reverse Sma I |
comE4 | tcccccggg ggacaagcaacaggtcatcccc | folC[10] forward Sma I |
comE6 | cgggatcc cggttgaatgcccggcaagcc | folC[10] reverse - Bam HI |
IL-1--fw | ggccgccaagatataactga | Porcine interleukin 1- |
IL-1--rev | ggacctctgggtatggctttc | Porcine interleukin 1- |
IL-6-fw | gacaaagccaccacccctaa | Porcine interleukin 6 |
IL-6-rev | ctcgttctgtgactgcagcttatc | Porcine interleukin 6 |
IL-8-fw | ttcgatgccagtgcataaata | Porcine interleukin 8 |
IL-8-rev | ctgtacaaccttctgcaccca | Porcine interleukin 8 |
IL-10-fw | gagaaactagggagcccctttg | Porcine interleukin 10 |
IL-10-rev | tggccacagctttcaagaatg | Porcine interleukin 10 |
IL-12-fw | ggagtataagaagtacagagtgg | Porcine interleukin 12-p40 |
IL-12-rev | gatgtccctgatgaagaagc | Porcine interleukin 12-p40 |
TNF-α-fw | cgcccacgttgtagccaatgt | Porcine TNF-α |
TNF-α-rev | cagatagtcgggcaggttgatctc | Porcine TNF-α |
IFN-γ-fw | caaagccatcagtgaactcatca | Porcine interferon-γ |
IFN-γ-rev | tctctggccttggaacatagtct | Porcine interferon-γ |
Orf2-fw | ctacggctggttcttctatcgaa | S. suis orf2 |
Orf2-rev | gcaatcggtgtcatgataaagg | S. suis orf2 |
folC-fw | gtttgtccgtccatcggttt | S. suis polyfolylpolyglutamate synthase |
Folc-rev | ctggtcggtcgcatagatga | S. suis polyfolylpolyglutamate synthase |
RecA-fw | ggtttgcaggctcgtatgatg | S. suis recombinase A |
RecA-rev | accaaacatgacaccgacttttt | S. suis recombinase A |
t488a | gaaaggtatagtttttagcaagtgga caaaatatatagtgtgtgatacaat | Promoter orf2 |
t488a_antisense | attgtatcacacactatatattttgt ccacttgctaaaaactatacctttc | Promoter orf2 |