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Table 1 Differentially expressed miRNAs identified to be commonly presented in w81-infected mouse lungs at both 1 dpi and 3 dpi

From: Differential microRNA expression following infection with a mouse-adapted, highly virulent avian H5N2 virus

miRNA

w81‡

1dpi

3dpi

Fold change*

P value§

Fold change

P value

let-7e-3p

2.6

0.008

2.3

0.006

miR-100-5p†

2.1

0.005

2.6

0

miR-10a-5p

2.1

0.009

2

0.002

miR-130a-5p†

3.4

0.036

3.2

0.01

miR-146b-3p†

3.1

0.014

4.7

0.003

miR-147-3p†

2.7

0.013

22.8

0.012

miR-148a-5p

2.3

0.029

2.4

0.002

miR-151-5p†

4.5

0.013

2.7

0.014

miR-155-3p†

2.3

0.015

3.5

0.016

miR-1843-3p

3.6

0.022

3.1

0.007

miR-1943-3p

4

0.01

4.3

0.049

miR-223-3p†

3.5

0.037

3.4

0.009

miR-28-3p

2

0.021

2.4

0.001

miR-301a-3p†

−6.9

0.019

−2

0.048

miR-3079-5p

2.8

0.035

2.4

0.034

miR-30b-5p

2

0.024

2.6

0.007

miR-30c-5p

2.5

0.038

2.2

0.024

miR-324-5p

2.1

0.02

3.1

0

miR-335-3p

2.1

0.004

2.7

0.003

miR-3475-3p

2.8

0.019

2.5

0.008

miR-365-3p

2.1

0.012

2.2

0.016

miR-495-3p†

2

0.042

2.9

0.01

miR-652-3p

3.9

0.042

2.8

0.011

miR-674-3p

2.5

0.019

2.2

0.001

miR-99b-5p

3.1

0.007

2.5

0

  1. ‡ global cellular miRNA expression patterns in mouse lungs infected with w81 were compared with those of uninfected control mouse lungs. Differentially expressed miRNAs with a p < 0.05 and fold change>2 are described.
  2. *Sequence reads were normalized to determine the number of transcripts per million (TPM) using the following formula: Normalized expression = Actual miRNA count/Total count of clean reads*1000000. Then, fold changes of miRNAs were evaluated using the following formula: Fold change = log2 (treatment/control).
  3. §P-value was calculated from the normalized expression values as previously described [66].
  4. †Significantly upregulated in both lungs infected with either wild type w81 or the mouse-adapted ma81 strain at all time points.