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Figure 1 | BMC Microbiology

Figure 1

From: Development of a transformation system for Aspergillus sojae based on the Agrobacterium tumefaciens-mediated approach

Figure 1

The ATMT donor vector pRM-eGFP. The transfer DNA region (T-DNA) consists of the phleomycin-resistance conferring gene (ble) which is under control of the A. nidulants trpC promoter (PtrpC) and trpC terminator (TtrpC). The enhanced green fluorescent protein (EGFP) reporter gene is under control of the constitutive A. nidulants gpdA promoter (PgpdA). The blue arrows indicate the target sites for the oligonucleotide primers BLE-F, BLE-R, EGFP-F and EGFP-R. OriV = replication origin; KanR = kanamycin resistance gene; TrfA = trans-acting gene trfA.

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BMC Microbiology

ISSN: 1471-2180

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