Figure 1

Regulation of vapA transcription and VapA expression in R. equi . (A) Western blot analysis performed with an anti-VapA antibody on cell extracts prepared from R. equi grown under conditions indicated in the Figure (B) β-galactosidase activity assays of P _vapA -lacZ fusions in the R. equi TKR255 strain grown at different temperatures and pH indicated in the Figure TKR255 was grown overnight at 30°C, diluted to an OD_60th0 of 0.05 in fresh medium at the pH indicated, and incubated at the temperature indicated. β-galactosidase activity was measured in Miller units and error bars represent standard deviations for each data set (n = 3). Data were evaluated for statistical significance using one-way ANOVA followed by the Dunnett s multiple comparison test, *p < 0.001.