production in Leishamania amazonensis promastigotes treated with 55, 139, 277, and 555 μM BZTS for up to 3 h using the fluorescence probe MitoSOX. Antimycin A (10 μM) was used as a positive control. Fluorescence was measured with a VICTOR X3 spectrofluorometer (Perkin-Elmer). The results are expressed as the mean ± standard error of MitoSOX oxidation (arbitrary units) from three independent experiments. *p ≤ 0.05, significant difference from negative control group.