The heat sensitive factor (HSF) of Yersinia ruckeriis produced by an alkyl sulphatase involved in sodium dodecyl sulphate (SDS) degradation but not in virulence

Table 2 Plasmids used in this study

Plasmid	Characteristics	Source or reference
pGBM5	Spc^r/Sm^r, lac promoter	[32]
pGBM5-yraS	pGBM5 harboring yraS gene	This study
pUC19	Ap^r, cloning vector	Pharmacia
pUT mini-Tn5 Km2	Ap^r, oriR6K, mobRP4, tnp, mini-Tn Km2 (Km^r)	[33]

ISSN: 1471-2180