Table 5 Plasmids used in this study
From: Clostridium difficilehas a single sortase, SrtB, that can be inhibited by small-molecule inhibitors
Plasmid | Description | Reference |
|---|---|---|
pQE30Xa- srtB | Codon optimized srtB, synthesized and cloned in pQE30xa | Obtained from Celtek Bioscience, LLC |
pET28a | Commercial protein expression vector | Provided by Neil Fairweather |
pET28a- srtB | Codon optimized srtB cloned in pET28a | This work |
pET28a- srtB ΔN26 | srtB with residues 2-25 replaced with a six-histidine tag | This work |
pET28a- srtB ΔN26,C209A | Same as above, with C209A substitution | This work |