Elevated recombinant clyA gene expression in the uropathogenic Escherichia coli strain 536, a clue to explain pathoadaptive mutations in a subset of extraintestinal E. coli strains

Table 4 Plasmids used in this work

Plasmid	Relevant characteristics	Reference/source
pBR322	Cloning vector, Tc^r	[55]
pGEM-T Easy	T-vector for cloning of PCR-fragments, Cb^r	Promega
pJON77	3.1-kb subclone of clyA in pSL1180, Cb^r	This work
pJON78	3.1-kb subclone of clyA in pKO3, Cm^r	This work
pJON176	clyA ⁺ kan in pJON78, Cm^r Km^r	This work
pKO3	Gene replacement vector, Cm^r	[56]
pMWK4	clyA::luxAB in pCH257, Cm^r	[8]
pAES1	pBR322, sfaX _II gene from sfa _II operon	[41]
pHMG95	pBR322, lacP(UV5)papI ⁺ clone	[57]
pSL1180	Cloning vector, Cb^r	[58]
pUC4K	Kanamycin resistance gene cartridge plasmid, Kan^r	[59]
pUC18	Cloning vector, Cb^r	[60]
pYMZ81	A 1.6-kb clyA locus in pUC18	[8]
pYMZ62	3.5-kb subclone of clyA in pUC18	[8]

ISSN: 1471-2180