Confirmation of pRS218 curing. A, Plasmid profiles of wtRS218 and RS218cured.
B, PCR amplification of selected pRS218 genes in wtRS218 and RS218cured. Lane 1,100 bp ladder; Lane 2, senB; Lane 3, scsD; Lane 4, transposase; Lane 5, traU; Lane 6, pRS218_113; Lane 7, ycfA; Lane 8, ompA. C, Growth of wtRS218 and RS218cured
E. coli in LB broth, M9 medium containing 10μgg/ml niacin broth (M9), and complete cell culture medium (CM).