Figure 1

Confocal laser scanning microscopy of P. gingivalis - F. nucleatum - S. gordonii community. S. gordonii cells (red, stained with hexidium iodide) were cultured on a glass plate. FITC-labeled F. nucleatum cells (green), followed by DAPI labeled P. gingivalis cells (blue), were reacted sequentially with the S. gordonii substratum. Bacterial accumulations were examined on a Bio-Rad Radiance 2100 confocal laser scanning microscope. A series of fluorescent optical x-y sections in the z-plane to the maximum vertical extent of the accumulation were collected with Laser Sharp software. Images were digitally reconstructed with Imaris software. Image is representative of three independent experiments.