Amounts of selected proteins representing different branches of metabolism. A, glycolysis/gluconeogenesis; B, TCA cycle; and C, amino acid degradation. Differential protein amounts 1 h after addition of glucose to exponentially growing cells are shown. The protein levels in the wild-type (1) and mutant (2) in the presence of glucose (green) were compared with the protein levels in the absence of glucose (red). In addition, the protein levels in the presence of glucose (3) in the wild-type (green) were compared to those in the mutant (red).