Table 1 Bacterial strains and plasmids used in this study

Strains

C. violaceum CV026

White indicator strain; cviI::Tn5 xylE; Amp^r, Kan^r, Str^r, Tet^s, Ery^s, Chl^s

E. coli CA027ZC09

The genomic clone generated from Ralstonia solanacearum GMI1000 for sequencing harbor plasmid pZC09 containing aac gene (RSc2547); Amp^r

E. coli DH10B

F^-mcrAΔ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 deoR recA 1 endA1 araΔ139 Δ(ara leu)7697 galU galK λ^-rpsL nupG; Str^r

E. coli BL21(DE3)

hsdS gal (λ cIts 857 ind1 Sam7 nin 5 lacUV5-T7 gene 1)

Candida tropicalis F-129

Test strain for the MIC of aculeacin A assay

Plasmids

pZC09

Plasmid generated from Ralstonia solanacearum GMI1000 for sequencing project from which the aac gene was amplified; Amp^r

pBBR1MCS-3

Mobilisable broad-host-range cloning vector; low copy number; mol, rep, lacZ; Tet^r

pS3aac

Transcriptional fusion of aac gene in pBBR1MCS-3; Tet^r

pET21a

Expression vector; T7 promoter; C-terminal HisTag; lacI; Amp^r

pET21aac

Translational fusion of aac gene in pET21a; Amp^r