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Table 1 Bacterial strains and plasmids used in this study

From: A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity

Strain or plasmid Genotype or Descriptiona Reference
C. violaceum CV026 White indicator strain; cviI::Tn5 xylE; Ampr, Kanr, Strr, Tets, Erys, Chls 27
E. coli CA027ZC09 The genomic clone generated from Ralstonia solanacearum GMI1000 for sequencing harbor plasmid pZC09 containing aac gene (RSc2547); Ampr INRA-CNRSb
E. coli DH10B F-mcrAΔ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 deoR recA 1 endA1 araΔ139 Δ(ara leu)7697 galU galK λ-rpsL nupG; Strr Invitrogen
E. coli BL21(DE3) hsdS gal (λ cIts 857 ind1 Sam7 nin 5 lacUV5-T7 gene 1) Novagen
Candida tropicalis F-129 Test strain for the MIC of aculeacin A assay 29
pZC09 Plasmid generated from Ralstonia solanacearum GMI1000 for sequencing project from which the aac gene was amplified; Ampr INRA-CNRSb
pBBR1MCS-3 Mobilisable broad-host-range cloning vector; low copy number; mol, rep, lacZ; Tetr 30
pS3aac Transcriptional fusion of aac gene in pBBR1MCS-3; Tetr This study
pET21a Expression vector; T7 promoter; C-terminal HisTag; lacI; Ampr Novagen
pET21aac Translational fusion of aac gene in pET21a; Ampr This study
  1. a Amp: ampicillin; Kan: kanamycin; Tet: tetracycline; Nal: nalidixic acid; Str:
  2. streptomycin; Chl: chloramphenicol; Ery: erythomycin
  3. b INRA-CNRS: Laboratoire de Biologie Moleculaire des Relations Plantes
  4. Microorganismes INRA-CNRS, France