piggyBac is an effective tool for functional analysis of the Plasmodium falciparumgenome

BMC Microbiology

Table 1 Doubling time estimation of P. falciparum mutant clones

Clone ID	Doubling time estimate (hours)	Standard error	95% CI		P value	t value	df
A5	22.07	0.26	21.53	22.60	0.00007	7.4656	7
B3	17.89	0.06	17.77	18.00	0.97376	-2.3316	7
B4	18.45	0.10	18.25	18.66	0.41380	0.2261	7
B7	19.70	0.17	19.34	20.06	0.00368	3.7297	7
E6	19.28	0.12	19.04	19.52	0.00565	3.4086	7
F3	21.98	0.17	21.64	22.33	0.00001	10.5459	7
F10	17.83	0.09	17.64	18.03	0.97735	-2.4318	7
G1	18.17	0.08	18.02	18.33	0.83353	-1.0400	7
H11	18.03	0.11	17.80	18.26	0.89928	-1.4098	7
WT	18.39	0.06	18.26	18.52	N/A: WT is reference

Doubling times for mutant parasite clones were estimated using their respective best-fit growth curves (See Fig. S1 in Additional file 2). Simple two-tailed t-test was then used to test the significance of differences in doubling time of mutant clones with wild type (WT) P. falciparum clones (average of three NF54 clones) as the reference. Significant P values, based on alpha = 0.05, are highlighted in bold.

ISSN: 1471-2180