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Table 1 Expression analysis of PCNA, POLD1, RFC and RPA using three different housekeeping controls.

From: Cervical cancer isolate PT3, super-permissive for adeno-associated virus replication, over-expresses DNA polymerase δ, PCNA, RFC and RPA

Probe set

Description

Gene symbol

PT3

Non-PT3

Fold Differences

   

ACTB

GAPDH

U133-A

ACTB

GAPDH

U133-A

ACTB

GAPDH

U133-A

201202_at

proliferating cell nuclear antigen

PCNA

13.4

13.5

13.7

11.7

11.8

12.3

3.2

3.2

2.6

203422_at

polymerase (DNA directed), delta 1

POLD1

11.1

11.2

11.3

9.9

10.0

10.2

2.2

2.3

2.2

204128_s_at

replication factor C (activator 1) 3, 38 kDa

RFC3

11.4

11.5

11.6

9.4

9.4

9.9

4.0

4.0

3.2

204127_at

replication factor C (activator 1) 3, 38 kDa

RFC3

12.3

12.3

12.5

10.7

10.7

11.2

3.0

3.0

2.5

204023_at

replication factor C (activator 1) 4, 37 kDa

RFC4

13.3

13.4

13.6

11.3

11.4

11.9

4.0

4.0

3.3

203209_at

replication factor C (activator 1) 5, 36.5 kDa

RFC5

11.4

11.4

11.6

10.0

10.1

10.5

2.6

2.6

2.1

201528_at

replication protein A1, 70 kDa

RPA1

11.9

12.0

-

10.8

10.9

-

2.1

2.1

-

201529_s_at

replication protein A1, 70 kDa

RPA1

12.3

12.4

-

11.2

11.3

-

2.0

2.0

-

201756_at

replication protein A2, 32 kDa

RPA2

12.5

12.6

12.7

10.9

11.0

11.5

2.9

2.9

2.3

  1. Three difference methods for data normalization using ACTB, GAPDH, and Affymetrix U-133A housekeeping genes, respectively, were utilized.