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Table 1 Expression analysis of PCNA, POLD1, RFC and RPA using three different housekeeping controls.

From: Cervical cancer isolate PT3, super-permissive for adeno-associated virus replication, over-expresses DNA polymerase δ, PCNA, RFC and RPA

Probe set Description Gene symbol PT3 Non-PT3 Fold Differences
    ACTB GAPDH U133-A ACTB GAPDH U133-A ACTB GAPDH U133-A
201202_at proliferating cell nuclear antigen PCNA 13.4 13.5 13.7 11.7 11.8 12.3 3.2 3.2 2.6
203422_at polymerase (DNA directed), delta 1 POLD1 11.1 11.2 11.3 9.9 10.0 10.2 2.2 2.3 2.2
204128_s_at replication factor C (activator 1) 3, 38 kDa RFC3 11.4 11.5 11.6 9.4 9.4 9.9 4.0 4.0 3.2
204127_at replication factor C (activator 1) 3, 38 kDa RFC3 12.3 12.3 12.5 10.7 10.7 11.2 3.0 3.0 2.5
204023_at replication factor C (activator 1) 4, 37 kDa RFC4 13.3 13.4 13.6 11.3 11.4 11.9 4.0 4.0 3.3
203209_at replication factor C (activator 1) 5, 36.5 kDa RFC5 11.4 11.4 11.6 10.0 10.1 10.5 2.6 2.6 2.1
201528_at replication protein A1, 70 kDa RPA1 11.9 12.0 - 10.8 10.9 - 2.1 2.1 -
201529_s_at replication protein A1, 70 kDa RPA1 12.3 12.4 - 11.2 11.3 - 2.0 2.0 -
201756_at replication protein A2, 32 kDa RPA2 12.5 12.6 12.7 10.9 11.0 11.5 2.9 2.9 2.3
  1. Three difference methods for data normalization using ACTB, GAPDH, and Affymetrix U-133A housekeeping genes, respectively, were utilized.