Oleate lipase activity in Gardnerella vaginalis and reconsideration of existing biotype schemes

BMC Microbiology

Table 1 Characteristics of Gardnerella vaginalis isolates on GVA: Biotypes, strain designations, growth and enzymatic activities

Isolate designation (Biotype)^A		48 hr growth GVA plates	Viability at 7 days On GVA plates^B	Aerobic Growth on EY	Lipase Egg yolk^C	Lipase with 4-MUO	Sialidase
0284	(1)	+	+	+	+	-	+
0287	(1)	+	+	+	+	-	+
0828E	(1)	+	+	+	+	-	-
0377	(1)	+	+	+	+	-	-
ATCC 14018	(1)	+	+	+	+	-	-
C31g 7571–2	(1)	+	+	+	+	-	-
ITP	(1)	+	+	+	+	-	-
PID 0286E	(1)	+	+	+	+	+	+
UPS 1388E	(1)	+	+	-	+	+	-
UPS1492	(1)	+	+	+	+	-	+
551	(2)	+	+	+	+	-	-
UPS1476	(2)	+	+	+	+	+	-
HPTN
208000837	(2)	+	+	+	+	-	+
UC781 64/20- LITTLE	(2)	+	+	+	+	+	+
UC781 64/20- BIG	(2)	+	+	+	+	+	+
UP548	(2)	+	+	-	+	-	+
UPS 754	(2)	+	+	+	+	-	-
055/15	(3)	+	+	+	+	+	-
UP537 E	(3)	+	+	+	+	-	-
RING52576	(4)	+	+	+	+	+	-
HPTN 213000700	(4)	+	+	+	+	-	+
UPS 1400E	(4)	-/scant^D	+	+	+	-	-
UPS 1398Ea	(5)	+	+	-	-	-	+
UPS 1483E	(5)	-	+	-	-	+	-
UPS 1500E	(5)	+	+	-	-	-	-
061/19 V5	(7)	+	+	+	-	+	+
HPTN008 000750	(7)	-	+	-	-	+	-
Up 582	(7)	+	+	+	-	+	-
UPS 1401 E	(7)	+	+	+	-	+	+
UPS 2050E	(7)	+	+	+	-	-	-
UPS1475	(7)	+	+	-	-	+	-

^AThe biotype of each isolate is given in parentheses.
^BTo accommodate those isolates demonstrating better growth anaerobically all strains were incubated in anaerobe jars. Aerobic organisms survived equally well under either incubation condition. Cultures were considered viable after 7 days if they were successfully subcultured to fresh GVA and blood agar plates.
^CAll strains were tested for lipase production on egg yolk agar under aerobic and anaerobic conditions; strains demonstrating growth aerobically yield identical lipase reaction when grown anaerobically. When strains did not grow aerobically on egg yolk plates the reactions indicated are taken from anaerobic incubation. Lipase reactions using 4-methylumbelliferone-oleate are given in parentheses.
^DSome organisms demonstrated poor growth when incubated in air plus 6% CO₂, these organisms all had excellent growth on GVA plates after anaerobic incubation.

ISSN: 1471-2180