Synergy between type 1 fimbriae expression and C3 opsonisation increases internalisation of E. coli by human tubular epithelial cells

Table 3 Phenotyping of E. coli blood isolates.

Strains	Internalisation rate (NHS/HIS)	Type 1- fimbriae	P- fimbriae	CNF1	Serum resistance	α-Haemolysin
J96	25	P	P	P	P	P
Internalised
B1	6.3	P	P	P	P	P
B2	33	P	P	P	P	P
B3	19	P	N	N	P	N
Non-internalised
B4	3.5	P	P	P	P	P
B5	3.3	P	P	N	P	P
B6	3.0	N	P	N	P	P
B7	1.2	N	P	N	P	P
B8	1.5	N	P	N	P	N
B9	2	N	P	N	P	N
B10	1.2	N	P	N	P	N
B11	0.7	N	N	P	P	P
B12	1.3	N	N	N	P	P
B13	1	N	N	N	P	N
B14	0.5	N	N	N	P	N
B15	2.2	N	N	N	P	N

In 15 blood isolates, bacterial virulence factors (including type-1, P fimbriae, CNF1, serum resistance, and α-Haemolysin) were examined to determine their correlation to C3-dependent internalisation (P positive, N negative). All experiments were repeated at least three times.

ISSN: 1471-2180