Strain/Target | Oligonucleotide (name and sequence), 5'→3' | Primer pair | Product size (bp) | |
---|---|---|---|---|
Nostoc punctiformeATCC 29133 | ||||
RT-Reaction | Â | Â | Â | Â |
hupW- antisense | HupW N R | TCA CAT CAT CGG GAA AGT CA | Â | Â |
Subsequent PCR | Â | Â | Â | Â |
hupW-antisense | HybD-RACE 1 | TTC TGG CAA AGC TTC CAG TT | L0373/L0372 | 808/1361 |
Npun_F0373-sense | L0373 | AAT TAT CTC CCT CGC GTT CC | HybD-RACE 1 | 808 |
NpF0372-sense | L0372 | TTG CCG ATG AAA CAA ATG AA | HybD-RACE 1 | 1361 |
Northern blot, probe | Â | Â | Â | Â |
hupW-antisense | HupW N R | TCA CAT CAT CGG GAA AGT CA | NB hupW N | 336 |
hupW-sense | NB hupW N L | TTG GTT GCG GAA ATC TCA AT | HupW N R | 336 |
5'RACE | Â | Â | Â | Â |
cDNA synthesis | HybD-RACE 1 | TTC TGG CAA AGC TTC CAG TT | Â | Â |
1 st PCR | HybD-RACE 2 | TGT TGG GCA ATG ATT ACA CCT | Â | Â |
2 nd PCR | HybD-RACE 3 | ATT GAG ATT TCC GCA ACC AA | Â | Â |
Nostoc sp. strain PCC 7120 – hupW | ||||
RT-Reaction | Â | Â | Â | Â |
hupW- antisense | NB HupW- AR | TGC TGT AGG CGT AAT CAT CG | Â | Â |
Subsequenct PCR | Â | Â | Â | Â |
hupW-antisense | Alr1422-23 R | TTT GTA AGC GTT GAG CGA TG | Alr1422-23 L | 490 |
Alr1422-sense | Alr1422-23 L | ACC GAA CTC CGC AGA AAC TA | Alr1422-23 R | 490 |
5'RACE | Â | Â | Â | Â |
cDNA synthesis | ALR1423 RACE 1b | GTT CCG AAC CAG TGG AAC TC | Â | Â |
1 st PCR | ALR1423 RACE 2 | TTT GTA AGC GTT GAG CGA TG | Â | Â |
2 nd PCR | ALR1423 RACE 3 | GAG ATT TCC GCA ACC GAT AA | Â | Â |
Nostoc sp. strain PCC 7120 – alr1422 | ||||
5'RACE | Â | Â | Â | Â |
cDNA synthesis | 5-1422-1 | CCTAAAGTCGGTGGAAAATCGGC | Â | Â |
1 st PCR | 5-1422-2 | TTCTTCCGTGACAAATCGTG | Â | Â |
2 nd PCR | 5-1422-3 | TTTTTGATGGACGGATGACA | Â | Â |
Nostoc sp. strain PCC 7120 – hoxW | ||||
Northern blot, probe | Â | Â | Â | Â |
hoxW-antisense | NB HoxW A R | AAA GCG ATC GCC TAT TTC AA | HoxW L | 316 |
hoxW-sense | HoxW L | AGG ACA ACG GAT AGC GAA TG | NB HoxW A R | 316 |
5'RACE | Â | Â | Â | Â |
cDNA synthesis | 5'RACE-1 HoxW/A | CAC AGC ACG ACG AAC AAG GCT CCA ACT TCA AAC CA | Â | Â |
1 st PCR-TAG | 5'RACE-TAG Hox/A | CAC AGC ACG ACG AAC AAG G | 5'RACE-polyG Hox/A | Â |
1 st PCR-PolyG | 5'RACE-polyG Hox/A | CAC AGC ACG ACG AAC AAG GGG GGG GGG GG | 5'RACE-TAG Hox/A | Â |