Virus associated mortality of Ae. aegypti HWE mosquitoes following infection by TE/3'J/B2 virus. A) Oral bloodmeal infection Mosquitoes were given an infectious oral bloodmeal containing 1 × 107 PFU of virus and kept at optimal rearing conditions. Mortality was monitored daily for a total of 21 days. n = 200 mosquitoes per group. B) Infection via intrathoracic injection Mosquitoes were injected with virus stock diluted to 1 × 107 PFU/ml and mortality was monitored daily. Day one mortality was not included. Black diamonds = Mock; Black circles = TE/3'2J; Black squares = TE/3'2J/GFP; Black triangles = TE/3'2J/B2. C) Determination of a mosquito 50 percent lethal dose for TE/3'2J-B2 infection. Groups of mosquitoes were intrathoracically injected with TE/3'2J/B2 virus diluted ten-fold and mortality was monitored daily. n = 50 mosquitoes/group. White bar indicates 50% mortality.