From: Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica
Oligo Name | Oligo Sequence | mRNA/cDNA section amplified (bp from ATG) | Total length of mRNA (bp) |
---|---|---|---|
Igl 5' F | GCTGTTCCACATTGTGCATCAGTTTCAAATG | 85–450 (Igl1), 85–459 (Igl2) | 3306 (Igl1), 3318 (Igl2) |
Igl 5' R | TTCTGCATGATCTTCTGTAGTTGCATTATCACATAAC | Â | Â |
Igl 3' F | TGAAGGCACTTCTACAGAAGATAATAAAAT | 2967–3166 (Igl1), 2979–3178 (Igl2) |  |
Igl 3' R | TATGTCTTGAACATGGAATACATGATC | Â | Â |
Igl1 F | TCTTGTAATAAGTTCCCGGAGCA | 634–841 (Igl1) |  |
Igl1 R | CATCAGAAACAGTACATCTTTTATTACATG | Â | Â |
Igl2 F | GTACTAAATACCCAGATCATTGTTCAAA | 643–841 (Igl2) |  |
Igl2 R | CATCAGAAACAGTACATCTTTTATTACATG | Â | Â |
URE3-BP 5' F | CCTGTAGCTAATTTCTGTTTATGGAATC | 10–155 | 663 |
URE3-BP 5' R | CTTGTATATTGATCTAATGGGATAGTGTTAAG | Â | Â |
URE3-BP Middle F | GATGAGAATTTTTGATACTGATTTTAATGGAC | 276–454 |  |
URE3-BP Middle R | GATTAATATAGAATCCAAGTTGTTGAAGAG | Â | Â |
URE3-BP 3' F | CTGTGATCTTAATTGTTGGATTG | 504–658 |  |
URE3-BP 3' R | CCAAGAGGGAAGTAACAACGT | Â | Â |
Actin F | GCACTTGTTGTAGATAATGGATCAGGAATG | variable (detects all family members/alleles) | variable |
Actin R | ACCCATACCAGCCATAACTGAAACG | Â | Â |
Jacob F | CAAAGGAGTTCAAATGGGATGTGTTAG | variable (detects all family members/alleles) | variable |
Jacob R | TTATTTGGTGTAGGAGTTGGTAATGGG | Â | Â |