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Table 2 Primer sequences and annealing temperatures (Real Time PCR)

From: Inducible expression of beta defensins by human respiratory epithelial cells exposed to Aspergillus fumigatusorganisms

Primers Sequences Conditions
hBD2f
hBD2r
5'-tatctcctcttctcgttcctcttc-3'
5'-ccacaggtgccaatttgtttatac-3'
40 cycles, 55°C,
2.5% DMSO
hBD9f
hBD9r
5'-ggcctaaatccaggtgtgaa-3'
5'-tcaaatgttggcaagtggag-3'
40 cycles, 55°C
GAPDHf
GAPDHr
5'-acccactcctccacctttgac-3'
5'-tccaccaccctgttgctgtag-3'
40 cycles, 55°C