Figure 1

Characterization of 9-species 'subgingival' in vitro biofilm model. (A) Time flow of biofilm formation. Data are means from triplicate biofilms and gained comparatively by culture analysis (left panel), FISH with taxa-specific 16S rRNA probes (central panel), or indirect IF with species-specific mAb (right panel, only four taxa). (B) Total bacteria and all individual taxa of 64.5 h biofilms enumerated by culture (upper panel) and FISH/IF(lower panel), assessing four completely independent experiments, each performed with triplicate biofilms. Culture data are expressed as CFU per biofilm, FISH/IF data as number of bacteria per biofilm. Box colors indicate Gram-negative (red) or Gram-positive (blue) bacteria. Abbreviations: TCC, total cell count; Crec, Campylobacter rectus; Fnuc, F. nucleatum subsp. vincentii; Pgin, Porphyromonas gingivalis; Pint, Prevotella intermedia; Tfor, Tannerella forsythia; Vdis, Veillonella dispar; Anae, Actinomyces naeslundii; Sint, Streptococcus intermedius; Sora, Streptococcus oralis.