Skip to main content

Table 1 Bacterial strains used in this study and MICs for KSM

From: The chlamydial functional homolog of KsgA confers kasugamycin sensitivity to Chlamydia trachomatisand impacts bacterial fitness

Strains Description Source or reference KSM MIC (μg ml-1)
E. coli    
   DH5α F-ϕ80Δ(lacZY-argF)U169 deoR recA1 endA1 phoA hsdR17 supE44 λ- thi-1 gyrA96 relA1 Δ(lacZ)M15 [56] NT
   BW25113 Δ(araD-araB)567 ΔlacZ4787 (::rrnB-3) λ - rph-1 Δ(rhaD-rhaB)568 hsdR514 [29] NT
   MC4100 F- araD139 Δ(argF-lac)U169 rpsL150 relA1 deoC1 rbsR fthD5301 fruA25 λ- [57] 150
   ATM809 MC4100 Δ ksgA::cat This work 2500
  ATM809 transformed with pRAK297 [Plac::E. coli ksgA in pGEMT] This work 200
  ATM809 transformed with pRAK325 [Plac:: C. psittaci 6BC UUG-ksgA in pGEMT] This work 1000
   ATM810 ATM809 att:: E.coli ksgA This work 200
   ATM812 ATM809 att:: C.trachomatis GUG-ksgA This work 800
   ATM811 ATM809 att:: C.trachomatis AUG-ksgA This work 400
   ATM815 ATM809 att:: C.trachomatis AUG-ksgAE9 This work 2500
C. psittaci 6BC strain 6BC T. Hatch 1100
   BCK1 Spontaneous KsmR variant of C. psittaci 6BC with A794G mutation in the 16S rRNA gene [13] >>5000
C. trachomatis L2 biovar lymphogranuloma venereum L2/434/Bu H. Caldwell 800
   L2 ksgAE9 C. trachomatis L2 ksgAE9 This work 3000
  1. NT; Not Tested