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Table 3 Real time RT-PCR showing PbGP43 transcript accumulation from three independent experiments, in which Pb339, Pb3 and Pb18 isolates were cultivated in F12/glc.

From: Polymorphism in the flanking regions of the PbGP43 gene from the human pathogen Paracoccidioides brasiliensis: search for protein binding sequences and poly(A) cleavage sites

Isolate

Samples

TA

N° of cells/mL

N° of days

Pb339

Exp1

3860 ± 51,5

9,2 × 106

4

 

Exp2

4443 ± 25,6

1,1 × 107

4

 

Exp3

10106 ± 108

1,6 × 107

4

Pb3

Exp1

41,6 ± 3,9

8,9 × 106

4

 

Exp2

55,5 ± 4,3

1 × 107

4

 

Exp3

51,66 ± 4,8

1,1 × 107

4

Pb18

Exp1

7,4 ± 0,8

1,4 × 107

6

 

Exp2

4,1 ± 0,5

1 × 107

6

 

Exp3

6,95 ± 0,5

1,2 × 107

6

  1. TA, relative number of transcript copies when compared with α-tubulin.
  2. Culture densities and ages are indicated.
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BMC Microbiology

ISSN: 1471-2180

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