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Figure 3 | BMC Microbiology

Figure 3

From: The hemolytic and cytolytic activities of Serratia marcescensphospholipase A (PhlA) depend on lysophospholipid production by PhlA

Figure 3

PLA1 and PLA2 activities of PhlA. PhlA activity was evaluated in a fluorescence enhancement assay using the following PLA fluorescence substrates: (A) bis-BODIPYFLC11-PC, (B) PED-A1, and (C) PED6. Fluorescence intensity was measured at 485 nm excitation and 530 nm emission using a fluorescence microplate reader (Appliskan; Thermo Electron Corporation). Open circles show His-PhlA; filled circles show PLA2 from bovine pancreas as a control. Values are averages ± SE from three independent experiments.

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