Helicobacter pylori lipopolysaccharide modification, Lewis antigen expression, and gastric colonization are cholesterol-dependent

Table 2 Enhancement of cell surface Lewis antigen expression by the growth of cultures in the presence of cholesterol.^a

	fold increase compared to parallel cholesterol-free culture
	Lewis X		Lewis Y
	mean ± SEM (n)	P value	mean ± SEM (n)	P value
26695	4.32 ± 0.36 (6)	0.0002	not done
SS1	not done		1.88 ± 0.08 (5)	0.0004
G27 wild type	2.85 ± 0.42 (8)	0.0033	2.22 ± 0.24 (8)	0.0016
G27 cgt::cat	3.69 ± 0.34 (5)	0.0013	2.88 ± 0.30 (5)	0.0034
G27 lpxE::cat	2.59 ± 0.50 (6)	0.025	2.47 ± 0.43 (7)	0.014

^a Lewis antigens were quantitated in replicate whole-cell ELISA analyses of paired samples grown in the presence or absence of 50 μg/ml cholesterol. The antigen load was 300 ng cellular protein per well. Ratios for plus:minus cholesterol were calculated from duplicate net absorbance readings in each assay, and ratios determined in five to eight independent ELISA runs were then averaged. P values were calculated in two-tailed Student t-tests for the null hypothesis that the ratio equals 1.

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