Figure 3

Kinetics of phosphate uptake. Initial uptake rates of ortho-phosphate (³³P, > 92.5 TBq mmol^-1) into LBT-grown whole cells of M. smegmatis mc²155 (solid squares) and the pitA deletion strain (open squares) were measured over 60 s at phosphate concentrations between 25 μM and 500 μM. Rates are expressed as nmol phosphate min^-1 mg mycobacterial protein extract^-1, and data are shown as the mean ± standard error of the mean from two to five independent measurements per point.