Table 3 Comparison of recombination efficiency using recombineering plasmids pACBSCE and pACBSR
From: Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia colistrains
KanR+SucI | % free of pDOC donor plasmid | % free of recombineering plasmid | ||||
|---|---|---|---|---|---|---|
Recombineering plasmid | pACBSCE | pACBSR | pACBSCE | pACBSR | pACBSCE | pACBSR |
MG1655 | 249 | 320 | 94 | 93 | 97 | 9 |
O157:H7 Sakai | 193 | 308 | 97 | 99 | 97 | 3 |
CFT073 | 174 | 234 | 91 | 93 | 99 | 0 |