Figure 1
From: Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia colistrains
The pDOC donor plasmids. Circular representation of the pEX100T plasmid showing the location of the origins of replication, the sacB gene and the ampicillin resistance gene. Below is a linear representation of the pDOC plasmid inserts, showing the I-SceI restriction sites, cloning regions (CR, CR1 and CR2), the Flp recognition sites flanking the kanamycin resistance cassette (KanR) and the location of the epitope tags in plasmids pDOC-H, pDOC-F, pDOC-P and pDOC-G.