Table 1 Fungal taxa identified on root tip samples from spruce and beech by sequencing of the ITS clone libraries of the pooled ECM tips and morphotyping/ITS sequencing of the individual ECM root tips.
Pooled ECM tips ITS cloning/ITS sequencing | Individual ECM tips Morphotyping/ITS sequencing | |||
|---|---|---|---|---|
Species name | Acc. n° | Identities (%) (Unite◆/NCBI○) | Acc. n° | Identities (%) (Unite◆/NCBI○) |
ECM from Picea abies: | ||||
Thelephora terrestris | EU427330.1 | 360/363 (100)○ | UDB000971 | 142/151 (94)◆ |
Cenococcum geophilum | UDB002297 | 375/379 (98)◆ | UDB002297 | 211/216 (97)◆ |
Clavulina cristata | UDB001121 | 375/375 (100)◆ | UDB 001121 | 281/289 (97)◆ |
Atheliaceae (Piloderma) sp | AY097053.1 | 343/362 (94)○ | EU597016.1 | 612/624 (98)○ |
Cortinarius sp 1 | AJ889974.1 | 361/367 (98)○ | UDB002224 | 232/242 (95)◆ |
Xerocomus pruinatus | UDB000018 | 348/351 (99)◆ | UDB 000016 | 692/696 (99)◆ |
Tomentelopsis submollis | AM086447.1 | 319/324 (98)○ | morphotyping only | |
Inocybe sp | AY751555.1 | 249/266 (93)○ | morphotyping only | |
Xerocomus badius | UDB000080 | 375/379 (98)◆ | UDB000080 | 400/417 (95)◆ |
Tylospora asterophora | UDB002469 | 353/354 (99)◆ | UDB002469 | 591/594 (99)◆ |
Tylospora fibrillosa | AF052563.1 | 405/408 (99)○ | AJ0534922.1 | 561/578 (97)○ |
Sebacina sp | not detected | UDB000975 | 162/168 (96)◆ | |
Lactarius sp 1 | not detected | morphotyping only | ||
ECM from Fagus sylvatica: | ||||
Pezizales sp | UDB002381 | 28/28 (100)◆ | DQ990873.1 | 602/646 (93)○ |
Sebacinaceae sp | EF619763.1 | 327/347 (94)○ | EF195570.1 | 495/497 (99)○ |
Laccaria amethystina | UDB002418 | 356/360 (98)◆ | UDB002418 | 276/277 (99)◆ |
Endophyte | AY268198.1 | 205/243 (84)○ | not detected | |
Inocybe napipes | UDB000017 | 292/294 (99)◆ | UDB000017 | 148/155 (95)◆ |
Xerocomus pruinatus | UDB000483 | 241/242 (99)◆ | UDB000483 | 279/288 (96)◆ |
Cortinarius sp 2 | UDB002410 | 416/437 (95)◆ | UDB002410 | 227/239 (95)◆ |
Cortinarius sp 3 | UDB002170 | 306/316 (96)◆ | UDB002445 | 57/59 (96)◆ |
Cortinarius tortuosus | UDB002164 | 279/284 (98)◆ | not detected | |
Russula puellaris | UDB000010 | 313/315 (99)◆ | UDB000010 | 246/247 (99)◆ |
Tomentellopsis submollis | UDB000198 | 272/273 (99)◆ | UDB000198 | 224/228 (98)◆ |
Laccaria laccata | UDB000104 | 322/327 (98)◆ | UDB000769 | 283/283 (100)◆ |
Cenococcum geophilum | not detected | UDB002297 | 216/222 (97)◆ | |
Amanita rubescens | not detected | morphotyping only | ||
Lactarius sp 2 | not detected | morphotyping only | ||
Tomentella sp | not detected | morphotyping only | ||