Hybridisation reactions of the species-specific fungal oligonucleotides. Reactions were tested by hybridising known fungal ITS pools to the phylochip. Vertical line indicates the fungal species used in the fungal ITS pools (hybridised probes), and the horizontal lines list the species-specific oligonucleotides. Grey boxes denote the positive hybridisation signals of an oligonucleotide obtained after threshold subtraction. The accompanying tree showing the phylogenetic relationship between tested fungal species was produced by the MEGAN programme. The size of the circle beside the genus name indicates the number of species of this genus used in the cross-hybridisation test.