Application and evaluation of the MLVA typing assay for the Brucella abortus strains isolated in Korea

BMC Microbiology

Table 4 Changes of 17 loci during in vitro serial passages

Locus	Number of passages that showed a change¹⁾				Change of the TRs copy number
	*B. abortus* 544	*B. abortus* 2308	*B. abortus* KBa019	*B. abortus* KBa011
Bruce 04	28	- ²⁾	-	-	An increase in one TRs
Bruce 16	28	-	-	-	An increase in one TRs
Hoof 3	29	-	-	-	An increase in one TRs
14 other loci	-	-	-	-	none

¹⁾ Four strains were sub-cultured to fresh media 30 times by serial passages at two- to three-day intervals
²⁾ No change after 30 passages

ISSN: 1471-2180