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Table 7 Summary of the performance of the biochemical, chromogenic and PCR methods for Cronobacter spp. identity confirmation.

From: Isolation of Cronobacter spp. (formerly Enterobacter sakazakii) from infant food, herbs and environmental samples and the subsequent identification and confirmation of the isolates using biochemical, chromogenic assays, PCR and 16S rRNA sequencing

Test Method Number of confirmed isolates Number of false positives Number of false negatives
API 20E 42 13 -
α-MUG 34 5 -
DFI 32 3 -
EsPM 33 7 3
Glu A PCR primers 26 3 6
Glu B*PCR primers 3 1 3
SG PCR primers 36 10 2
SI PCR primers 36 10 3
Ska PCR primers 25 - 4
OmpA PCR primers 27 - 2
Zpx 94 bp PCR primers 32 13 10
Zpx 350 bp PCR primers 32 6 3
  1. *: Only 9 samples were tested using these primers