Skip to main content

Table 1 Oligonucleotide nested primer sequences for the amplification and sequencing of the seven loci from genes in C. sakazakii and C. malonaticus, with gene number and location of genes within the genome of the C. sakazakii strain ATCC BAA-894.

From: Multilocus sequence typing of Cronobacter sakazakii and Cronobacter malonaticus reveals stable clonal structures with clinical significance which do not correlate with biotypes

     Locus Primers (5'→3')
Gene
(Gene label)
Putative Gene Product Chromosome location (bp) Gene Size (bp) Amplification Sequencing
atpD ATP synthase β chain 3,689,177 - 3,690,559 1,382 CGACATGAAAGGCGACAT CGAAATGACCGACTCCAA
(ESA_04006)     TTAAAGCCACGGATGGTG GGATGGCGATGATGTCTT
fusA Elongation factor 3,275,843 - 3,277,957 2,114 GAAACCGTATGGCGTCAG GCTGGATGCGGTAATTGA
(ESA_04401)     AGAACCGAAGTGCAGACG CCCATACCAGCGATGATG
glnS Glutaminyl-tRNA 660,368 - 662,035 1,667 GCATCTACCCGATGTACG GGGTGCTGGATAACATCA
(ESA_02658) synthetase    TTGGCACGCTGAACAGAC CTTGTTGGCTTCTTCACG
gltB Glutamate synthase 3,538,713 - 3,542,921 4,208 CATCTCGACCATCGCTTC GCGAATACCACGCCTACA
(ESA_03606) large subunit    CAGCACTTCCACCAGCTC GCGTATTTCACGGAGGAG
gyrB DNA gyrase B 3,719,848 - 3,722,262 2,414 TGCACCACATGGTATTCG CTCGCGGGTCACTGTAAA
(ESA_03973)     CACCGGTCACAAACTCGT ACGCCGATACCGTCTTTT
infB Translation initiation 4,139,051 - 4,141,762 2,711 GAAGAAGCGGTAATGAGC TGACCACGGTAAAACCTC
(ESA_03561) factor IF-2    CGATACCACATTCCATGC GGACCACGACCTTTATCC
pps Phosphoenol-pyruvate 1,218,599 - 1,220,977 2,378 GTCCAACAATGGCTCGTC ACCCTGACGAATTCTACG
(ESA_02102) synthase    CAGACTCAGCCAGGTTTG CAGATCCGGCATGGTATC