Table 1 Oligonucleotide nested primer sequences for the amplification and sequencing of the seven loci from genes in C. sakazakii and C. malonaticus, with gene number and location of genes within the genome of the C. sakazakii strain ATCC BAA-894.
|  |  |  |  | Locus Primers (5'→3') | |
|---|---|---|---|---|---|
Gene (Gene label) | Putative Gene Product | Chromosome location (bp) | Gene Size (bp) | Amplification | Sequencing |
atpD | ATP synthase β chain | 3,689,177 - 3,690,559 | 1,382 | CGACATGAAAGGCGACAT | CGAAATGACCGACTCCAA |
(ESA_04006) | Â | Â | Â | TTAAAGCCACGGATGGTG | GGATGGCGATGATGTCTT |
fusA | Elongation factor | 3,275,843 - 3,277,957 | 2,114 | GAAACCGTATGGCGTCAG | GCTGGATGCGGTAATTGA |
(ESA_04401) | Â | Â | Â | AGAACCGAAGTGCAGACG | CCCATACCAGCGATGATG |
glnS | Glutaminyl-tRNA | 660,368 - 662,035 | 1,667 | GCATCTACCCGATGTACG | GGGTGCTGGATAACATCA |
(ESA_02658) | synthetase | Â | Â | TTGGCACGCTGAACAGAC | CTTGTTGGCTTCTTCACG |
gltB | Glutamate synthase | 3,538,713 - 3,542,921 | 4,208 | CATCTCGACCATCGCTTC | GCGAATACCACGCCTACA |
(ESA_03606) | large subunit | Â | Â | CAGCACTTCCACCAGCTC | GCGTATTTCACGGAGGAG |
gyrB | DNA gyrase B | 3,719,848 - 3,722,262 | 2,414 | TGCACCACATGGTATTCG | CTCGCGGGTCACTGTAAA |
(ESA_03973) | Â | Â | Â | CACCGGTCACAAACTCGT | ACGCCGATACCGTCTTTT |
infB | Translation initiation | 4,139,051 - 4,141,762 | 2,711 | GAAGAAGCGGTAATGAGC | TGACCACGGTAAAACCTC |
(ESA_03561) | factor IF-2 | Â | Â | CGATACCACATTCCATGC | GGACCACGACCTTTATCC |
pps | Phosphoenol-pyruvate | 1,218,599 - 1,220,977 | 2,378 | GTCCAACAATGGCTCGTC | ACCCTGACGAATTCTACG |
(ESA_02102) | synthase | Â | Â | CAGACTCAGCCAGGTTTG | CAGATCCGGCATGGTATC |