Immunoblot detection of intra- and extra-cellular CDT of C. jejuni. Immunoblot analyses of samples from C. jejuni wild type strains 81-176 (lanes 1-4) and the cdtA::km mutant (lanes 5-8). Samples: 1&5; whole cells (WC), 2&6; supernatants 1 (S1), 3&7; supernatants 2(S2), 4&8; OMVs, (A) Immunoblot detection with anti-CdtA polyclonal antiserum, (B) immunodetection with anti-CdtB polyclonal antiserum. (C) immunoblot detection with anti-CdtC polyclonal antiserum. (D) immunoblot detection with anti-Omp50 polyclonal antiserum.