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Table 1 Bacterial strains and plasmids.

From: Environmental and intracellular regulation of Francisella tularensis ripA

Strains or Plasmid Description Source or Reference
Bacteria   
Francisella tularensis LVS F. tularensis live vaccine strain CDC, Atlanta, GA
   ripA::Tn5 Tn5 ripA transposon mutant [21]
   ripA::pBSK Φ(ripA'-lacZ)1 Plasmid cointegrate This work
   ripA:: pBSK Φ(ripA'-lacZ)2 Plasmid cointegrate This work
   iglA:: pBSK Φ(iglA'-lacZ) Plasmid cointegrate This work
   Φ(ripA'-TC) Exchanged allele This work
   ΔmglA Inframe deletion of mglA This work
   ΔmglA ripA:: pBSK Φ(ripA'-lacZ)2 Plasmid cointegrate This work
   ΔmglA iglA:: pBSK Φ(iglA'-lacZ) Plasmid cointegrate This work
   ΔsspA Inframe deletion of sspA This work
   ΔsspA ripA:: pBSK Φ(ripA'-lacZ)2 Plasmid cointegrate This work
   ΔsspA iglA:: pBSK Φ(iglA'-lacZ) Plasmid cointegrate This work
   ΔmglA ΔsspA Inframe gene deletions This work
   ΔmglA ΔsspA ripA:: pBSK Φ(ripA'-lacZ)2 Plasmid cointegrate This work
   ΔmglA ΔsspA iglA:: pBSK Φ(iglA'-lacZ) Plasmid cointegrate This work
Plasmids   
   pBSK bla lacZ pBluescript cloning vector Stratagene
   pBSK lacZ aphA1 bla Transcriptional lacZ fusion This work
   pBSK lacZ cat bla Translational lacZ fusion This work
   pBSK Φ(ripA'-lacZ)2 aphA1 bla Francisella suicide vector This work
   pBSK Φ(ripA'-lacZ)1 cat Francisella suicide vector This work
   pBSK Φ(iglA'-lacZ)2 aphA1 Francisella suicide vector This work
   pMP590 Francisella sacB suicide vector [47]
   pMP590 ΔmglA mglA allelic exchange vector This work
   pMP590 ΔsspA sspA allelic exchange vector This work
   pMP590 Φ(ripA'-TC) Φ(ripA'-TC) suicide vector This work
   pMP633 Francisella shuttle vector [47]
   pMP633 mglA+ mglA+ with native promoter This work
   pMP633 sspA+ sspA+ with native promoter This work
   pKK MCS Francisella shuttle vector [21]
   pKK MCS Φ(ripA'-lacZ)1 translational fusion This work
   pKK MCS Φ(ripA'-lacZ)1a -10 mutation This work
   pKK MCS Φ(ripA'-lacZ)1b RBS mutation This work
   pKK MCS Φ(ripA'-lacZ)1c lacZ frameshift This work
   pKK MCS Φ(ripA'-lacZ)1d ripA core promoter This work
   pKK MCS Φ(ripA'-lacZ)2 transcriptional fusion This work
   pKK MCS Φ(ripA'-TC) ripA-CT tetracysteine tag fusion This work