From: Environmental and intracellular regulation of Francisella tularensis ripA
Strains or Plasmid | Description | Source or Reference |
---|---|---|
Bacteria | Â | Â |
Francisella tularensis LVS | F. tularensis live vaccine strain | CDC, Atlanta, GA |
   ripA::Tn5 | Tn5 ripA transposon mutant | [21] |
   ripA::pBSK Φ(ripA'-lacZ)1 | Plasmid cointegrate | This work |
   ripA:: pBSK Φ(ripA'-lacZ)2 | Plasmid cointegrate | This work |
   iglA:: pBSK Φ(iglA'-lacZ) | Plasmid cointegrate | This work |
   Φ(ripA'-TC) | Exchanged allele | This work |
   ΔmglA | Inframe deletion of mglA | This work |
   ΔmglA ripA:: pBSK Φ(ripA'-lacZ)2 | Plasmid cointegrate | This work |
   ΔmglA iglA:: pBSK Φ(iglA'-lacZ) | Plasmid cointegrate | This work |
   ΔsspA | Inframe deletion of sspA | This work |
   ΔsspA ripA:: pBSK Φ(ripA'-lacZ)2 | Plasmid cointegrate | This work |
   ΔsspA iglA:: pBSK Φ(iglA'-lacZ) | Plasmid cointegrate | This work |
   ΔmglA ΔsspA | Inframe gene deletions | This work |
   ΔmglA ΔsspA ripA:: pBSK Φ(ripA'-lacZ)2 | Plasmid cointegrate | This work |
   ΔmglA ΔsspA iglA:: pBSK Φ(iglA'-lacZ) | Plasmid cointegrate | This work |
Plasmids | Â | Â |
   pBSK bla lacZ | pBluescript cloning vector | Stratagene |
   pBSK lacZ aphA1 bla | Transcriptional lacZ fusion | This work |
   pBSK lacZ cat bla | Translational lacZ fusion | This work |
   pBSK Φ(ripA'-lacZ)2 aphA1 bla | Francisella suicide vector | This work |
   pBSK Φ(ripA'-lacZ)1 cat | Francisella suicide vector | This work |
   pBSK Φ(iglA'-lacZ)2 aphA1 | Francisella suicide vector | This work |
   pMP590 | Francisella sacB suicide vector | [47] |
   pMP590 ΔmglA | mglA allelic exchange vector | This work |
   pMP590 ΔsspA | sspA allelic exchange vector | This work |
   pMP590 Φ(ripA'-TC) | Φ(ripA'-TC) suicide vector | This work |
   pMP633 | Francisella shuttle vector | [47] |
   pMP633 mglA+ | mglA+ with native promoter | This work |
   pMP633 sspA+ | sspA+ with native promoter | This work |
   pKK MCS | Francisella shuttle vector | [21] |
   pKK MCS Φ(ripA'-lacZ)1 | translational fusion | This work |
   pKK MCS Φ(ripA'-lacZ)1a | -10 mutation | This work |
   pKK MCS Φ(ripA'-lacZ)1b | RBS mutation | This work |
   pKK MCS Φ(ripA'-lacZ)1c | lacZ frameshift | This work |
   pKK MCS Φ(ripA'-lacZ)1d | ripA core promoter | This work |
   pKK MCS Φ(ripA'-lacZ)2 | transcriptional fusion | This work |
   pKK MCS Φ(ripA'-TC) | ripA-CT tetracysteine tag fusion | This work |