Use of in vivo -induced antigen technology (IVIAT) for the identification of Streptococcus suis serotype 2 in vivo -induced bacterial protein antigens

Table 1 Bacterial strains and plasmids used in this study

Strains or plasmids	Serotype, Genotype and/or phenotype	Reference/source
Strains
HA9801	serotype 2;cps2J+, mrp+, ef+, sly+, gapdh+, gdh+, orf2+	Jiangsu outbreak SS2 isolate, 1998, China
ZY05719	serotype 2; cps2J+, mrp+, ef+, sly+, gapdh+, gdh+, orf2+	Sichuan outbreak SS2 isolate, 2005, China
T15	serotype 2; cps2J+, mrp-, ef-	The Netherlands
Plasmids
pET30(abc)	Expression vectors allowing cloning of fragments in each of three reading frames; Kan^r	Novagen
pMRP	pET30(a) with partial mrp gene amplified from strain ZY05719, and cloned into EcoRI and XhoI sites, in vector, Kan^r	This work
pEF	pET30(a) with partial ef gene amplified from strain ZY05719, and cloned into EcoRI and XhoI sites, in vector, Kan^r	This work
pGAPDH	pET32(a) with partial gapdh gene amplified from strain ZY05719, and cloned into BamHI and SalI sites, in vector, Amp^r	Previous work