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Table 1 Bacterial strains and plasmids used in this study

From: Use of in vivo-induced antigen technology (IVIAT) for the identification of Streptococcus suis serotype 2 in vivo-induced bacterial protein antigens

Strains or plasmids Serotype, Genotype and/or phenotype Reference/source
Strains   
HA9801 serotype 2;cps2J+, mrp+, ef+, sly+, gapdh+, gdh+, orf2+ Jiangsu outbreak SS2 isolate, 1998, China
ZY05719 serotype 2; cps2J+, mrp+, ef+, sly+, gapdh+, gdh+, orf2+ Sichuan outbreak SS2 isolate, 2005, China
T15 serotype 2; cps2J+, mrp-, ef- The Netherlands
Plasmids   
pET30(abc) Expression vectors allowing cloning of fragments in each of three reading frames; Kanr Novagen
pMRP pET30(a) with partial mrp gene amplified from strain ZY05719, and cloned into EcoRI and XhoI sites, in vector, Kanr This work
pEF pET30(a) with partial ef gene amplified from strain ZY05719, and cloned into EcoRI and XhoI sites, in vector, Kanr This work
pGAPDH pET32(a) with partial gapdh gene amplified from strain ZY05719, and cloned into BamHI and SalI sites, in vector, Ampr Previous work
  1. Kanr, kanamycin-resistant; Ampr, ampicillin-resistant.