Strains
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HA9801
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serotype 2;cps2J+, mrp+, ef+, sly+, gapdh+, gdh+, orf2+
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Jiangsu outbreak SS2 isolate, 1998, China
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ZY05719
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serotype 2; cps2J+, mrp+, ef+, sly+, gapdh+, gdh+, orf2+
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Sichuan outbreak SS2 isolate, 2005, China
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T15
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serotype 2; cps2J+, mrp-, ef-
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The Netherlands
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Plasmids
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pET30(abc)
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Expression vectors allowing cloning of fragments in each of three reading frames; Kanr
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Novagen
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pMRP
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pET30(a) with partial mrp gene amplified from strain ZY05719, and cloned into EcoRI and XhoI sites, in vector, Kanr
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This work
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pEF
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pET30(a) with partial ef gene amplified from strain ZY05719, and cloned into EcoRI and XhoI sites, in vector, Kanr
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This work
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pGAPDH
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pET32(a) with partial gapdh gene amplified from strain ZY05719, and cloned into BamHI and SalI sites, in vector, Ampr
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Previous work
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