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Table 1 Biological material used in this work.

From: Identification of two GH18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete Aphanomyces astaci

Species Isolate: reference Origin (year, location) Issue addressed
A. astaci type 1 L1 Astacus astacus
(1962, Sweden)
CHI, MCA, TaqMan
A. astaci type 1 Ra A. astacus
(1973, Sweden)
CHI
A. astaci type 1 Sv A. astacus
(1970, Sweden)
CHI, MCA, TaqMan
A. astaci type 2 A. astacus
(1974, Sweden)
CHI, Chi activity, Western, PCR
A. astaci type 2 Ti A. astacus
(1970, Sweden)
CHI
A. astaci type 2 Yx A. astacus
(1973, Sweden)
CHI
A. astaci type 3 Kv1 Pacifastacus leniusculus
(1978, Sweden)
CHI
A. astaci type 4 Pc Procambarus clarkii
(1992, Sweden)
CHI
A. astaci GB04
(CBS 121.537)
P. leniusculus
(2004, Ganaubach, Austria)
CHI, PHYLO, RACE, GX, MCA, TaqMan
A. astaci GKS07
(CBS 121.538)
A. astacus
(2007, Gleinkersee, Austria)
PHYLO
A. astaci Z12
(CBS 117.160)
P. leniusculus
(2004, Zöbernbach, Austria)
PHYLO
A. frigidophilus NJM 9665
(ATCC 204464): [6, 61]
egg of Onchorhynchus masou (1996, Japan) CHI, MCA, TaqMan
A. frigidophilus SAP472: [30] Austropotamobius pallipes (2008, Spain) CHI
A. invadans WIC: [6] Brevoortia tyrannus
(2004, USA)
CHI, MCA, TaqMan
A. laevis CBS 107.52 unknown
(1952, unknown)
CHI, MCA, TaqMan
A. helicoides CBS 210.82 unknown
(1982, former USSR)
CHI, MCA, TaqMan
A. repetans LK29 P. leniusculus
(2004, Leithakanal, Austria)
CHI, PHYLO
A. irregularis CBS 278.81 pond
(1981, The Netherlands)
CHI, MCA, TaqMan
Achlya racemosa CBS 578.67 unknown
(1967, Great Britain)
CHI
Leptolegnia caudata CBS 680.69 unknown
(1969, Canada)
CHI, MCA, TaqMan
Saprolegnia parasitica CBS 540.67 fish hatchery
(1967, Great Britian)
CHI
Aspergillus sp. not assigned horse food
(2004, Vienna, Austria)
MCA
Fusarium solani CBS 181.29 unknown
(1929, Germany)
CHI
Trichosporon cutaneum DSM 70675 sulfite liquor waste CHI
  1. Western: western-blot analysis, CHI: partial sequencing of homologous chitinase gene(s), RACE: rapid amplification of cDNA ends, PHYLO: determination of ITS nrDNA sequences for phylogenetic analysis, GX: temporal gene expression of Chi2 and Chi3, MCA: qPCR/MCA for qualitative detection of A. astaci, TaqMan: TaqMan qPCR