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Table 2 Primers used in this studya

From: Extracellular secretion of Carocin S1 in Pectobacterium carotovorum subsp. carotovorumoccurs via the type III secretion system integral to the bacterial flagellum

Primer   Sequence (5'→3')
PR-1 ......... 5'-GCCGAAGAGAACACAGATTTAGCCCA
PR-2 ......... 5'-CCGCACGATGAAGAGCAGAAGTT
PR-3 ......... 5'-CAGATCTCTGGAAAACGGGAAAGG
PF-1 ......... 5'-AGAGAACACAGATTTAGCCCAGTCGG
PF-2 ......... 5'-CCGCACGATGAAGAGCAGAAGTTAT
PF-3 ......... 5'-GATCCTGGAAAACGGGAAAGGTTC
TH12-2F1 ......... 5'-GATGGTGAAATTGGCAGAAAC
TH12-2F2 ......... 5'-GGACATTAGTCCGGTTTGTTG
TH12-2R1 ......... 5'-CAACAAACCGGACTAATGTCC
TH12-2R2 ......... 5'-GTTTCTGCCAATTTCACCATC
N-1 ......... 5'-NGTCGA(G/C)(A/T)GANA(A/T)GAA
N-2 ......... 5'-GTNCGA(C/G)(A/T)CANA(A/T)GTT
N-3 ......... 5'-(A/T)GTGNAG(A/T)ANCANAGA
P-3 ......... 5'-CTCGACGTTGTCACTGAAGCGGGAAG
P-4 ......... 5'-AAAGCACGAGGAAGCGGTCAGCCCAT
DY-SR1 ......... 5'-GAAATCGATCACCGCCTTCACAC
DY-SF1 ......... 5'-AAAGAATTCTTCAGTCGCGTTG
flhA-sen ......... 5'-TCACTCAACGTTGCATCTAC
flhA-anti ......... 5'-CAAGATGTTGGCCAACAGATG
fliC-sen ......... 5'-TCGGTGCGAATGATGGTG
fliC-anti ......... 5'-AACGCAGCAGTGACAGC
fliC-Fu-sen ......... 5'-TGGTTTTATCCACGACTCAC
fliC-Fu-anti ......... 5'-ATGCAGCAGGATCCAGAAC
flhA-Fu-sen ......... 5'-TCACTCAAGCTTGCATCTAC
flhA-Fu-anti ......... 5'-CGGATTGTCGACTAGCTGG
  1. a All primers were purchased from MDE Bio Inc., Taipei, Taiwan