Effect of silencing several An. gambiae (G3) genes on parasite P. falciparum infection. Effect of silencing arginine kinase (ArgK) (Panel A), heat shock cognate 3 (Hsc-3) (Panel B), solute transporter (Sol. Trsp.) (Panel C), glutathione-S-transferase theta-1 (GSTT1) (Panel D), oxidation resistance gene 1 (OXR1) (Panel E) tetraspanin (Tetrasp.) (Panel F), and glutathione-S-transferase theta-2 (GSTT2) (Panel G) on P. falciparum infection. The number of P. falciparum oocysts present was determined by directly counting mercurochrome-stained parasites 7–8 days post infection. The dots represent the number of parasites present on individual midguts, and the median number of oocysts is indicated by the horizontal line. Distributions are compared using the Kolmogorov-Smirnov test; n = number of mosquitoes; P values lower than 0.05 are considered to be significantly different.