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Table 3 Comparison of phage titer determinations with DLA and with PAMA using different antibiotics

From: The use of antibiotics to improve phage detection and enumeration by the double-layer agar technique

 

DLA

AMP [0.5]

CEF [0.06]

TET [1.5]

 

phi PVP-SE1

PFUs (average ± SD)

14 ± 5

55 ± 10

53 ± 11

58 ± 10

SD %

38

19

21

17

 

phi PVP-SE2

PFUs (average ± SD)

54 ± 4

54 ± 5

48 ± 11

51 ± 3

SD %

8

8

23

6

  1. DLA: classical Double-Layer Agar technique; AMP [0.5]: PAMA with 0.5 mg/l ampicillin; CEF [0.06]: PAMA with 0.06 mg/l cefotaxime; TET [1.5]: PAMA with 1.5 mg/l tetracycline.