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Table 3 Comparison of phage titer determinations with DLA and with PAMA using different antibiotics

From: The use of antibiotics to improve phage detection and enumeration by the double-layer agar technique

  DLA AMP [0.5] CEF [0.06] TET [1.5]
  phi PVP-SE1
PFUs (average ± SD) 14 ± 5 55 ± 10 53 ± 11 58 ± 10
SD % 38 19 21 17
  phi PVP-SE2
PFUs (average ± SD) 54 ± 4 54 ± 5 48 ± 11 51 ± 3
SD % 8 8 23 6
  1. DLA: classical Double-Layer Agar technique; AMP [0.5]: PAMA with 0.5 mg/l ampicillin; CEF [0.06]: PAMA with 0.06 mg/l cefotaxime; TET [1.5]: PAMA with 1.5 mg/l tetracycline.