Figure 3

YbaB _Ec and YbaB _Hi are DNA-binding proteins. (A) Representative EMSA using labeled probe b-WT and increasing concentrations of recombinant YbaB_Ec. Lane 1 lacked YbaB_Ec, and lanes 2 through 12 contained 0.14, 0.21, 0.47, 0.93, 1.4, 1.8, 2.3, 4.7, 7.0, 9.4 or 12 μg/ml YbaB_Ec, respectively. (B) Representative EMSA using labeled probe b-WT and increasing concentrations of recombinant YbaB_Hi. Lane 1 lacked YbaB_Hi, and lanes 2 through 12 contained 0.18, 0.26, 0.59, 1.2, 1.8, 2.3, 2.9, 5.9, 8.8, 12 or 15 μg/ml YbaB_Hi, respectively.