Silver-stained of proteinase-K digested whole cell lysate from H. pylori wild-type and isogenic mutants. (A) Lanes 1–7 were all loaded with 2.5 × 108 proteinase K-digested bacteria (~130 μg total protein). Lane 1, 26695; lane 2, wild-type; lane 3, imp/ostA single mutant strain; lane 4, imp/ostA complementation strain; lane 5, msbA single mutant strain; lane 6, msbA complementation strain; lane 7,imp/ostA and msbA double mutant strain. Molecular weights of the prestained markers are indicated. (B-C) Immunoblots of LPS from H. pylori with anti-Lea or anti-Leb monoclonal antibodies. (B) anti-Lea (1:3000) as the primary antibody and anti-mouse IgG (1:5000) as the secondary antibody, or (C) anti-Leb (1:3000) as the primary antibody and anti-mouse IgG (1:5000) as the secondary antibody.