Figure 2
Sensitivity of Multiplex PCR amplifying simultaneously Cp. abortus AB7, Cp. pecorum iB1 and C. burnetii Nine Miles reference strains. Lane 1: 100-bp ladder; lane 2–7: variation of total genomic DNA amount isolated from the three bacteria (105, 104, 103, 102, 50 and 10 genome copies per PCR reaction); lane 8: Negative control without DNA.