Figure 4

The effect of LPS on Hs294T human melanoma migration on fibronectin. The insert: the 8-μm 0.3-cm² membrane was covered with fibronectin. Hs294T melanoma cells were applied at 1 × 10⁵ cells per insert in DMEM. LPS was applied as a dose gradient (10 U/ml equals 0.25 ng/ml). The concentration of the attracting agent FBS in the lower section of the migration chamber was 7.3–7.5%. Migration was carried out for 1 h 20 min at 37°C in CO₂. The cells were stained and counted under light microscopy on the whole membrane. The mean number of cells per membrane (bars) and SD (lines) are presented.