Figure 2

The effect of LPS on B16 mouse melanoma migration on fibronectin. The insert: an 8-μm 0.3-cm² membrane was covered with fibronectin. B16 melanoma cells were applied at 5 × 10⁵ cells per insert in DMEM. LPS was applied as a dose gradient (10 U/ml, equal to 0.25 ng/ml). The concentration of the attracting agent FBS in the lower section of the migration chamber was 7.3–7.5%. Migration was carried out for 2 h at 37°C in CO₂. The cells were stained and counted under light microscopy on the whole membrane. The mean number of cells per membrane (bars) and SD (lines) are presented.