Characterization of Zur-dependent genes and direct Zur targets in Yersinia pestis

BMC Microbiology

Table 2 Promoter activity determined by LacZ reporter fusion analysis

LacZ fusion	Plasmid copy number (WT/*Δzur*)	Normalized Miller Units	Fold change (*Δzur*/WT)
WT-znuC	5.45 ± 0.73	6343.95 ± 237.68	2.60
Δzur-znuC		16507.10 ± 344.19
WT-znuA	11.52 ± 0.92	12281.64 ± 428.30	7.77
Δzur-znuA		95498.09 ± 1962.30
WT-ykgM	3.09 ± 0.88	118.64 ± 6.77	4.71
Δzur-ykgM		559.29 ± 28.14

Notes: The promoter DNA regions upstream znuC, znuA and ykgM were cloned into the pRS551 plasmid, respectively, to fuse with the promoterless lacZ gene. β-Galactosidase activity (miller units) was detected to represent the promoter activity. Copy number of recombinant pRS551 was determined by real-time quantitative PCR, with the primers specific for the borne lacA gene. The detecting fold change of plasmid copy number was set to be 1 to generate a normalization factor that was subsequently used for generating the normalized fold change of promoter activity (miller units) in WT in relative to Δzur.

ISSN: 1471-2180