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Table 2 Promoter activity determined by LacZ reporter fusion analysis

From: Characterization of Zur-dependent genes and direct Zur targets in Yersinia pestis

LacZ fusion Plasmid copy number (WT/Δzur) Normalized Miller Units Fold change (Δzur/WT)
WT-znuC 5.45 ± 0.73 6343.95 ± 237.68 2.60
Δzur-znuC   16507.10 ± 344.19  
WT-znuA 11.52 ± 0.92 12281.64 ± 428.30 7.77
Δzur-znuA   95498.09 ± 1962.30  
WT-ykgM 3.09 ± 0.88 118.64 ± 6.77 4.71
Δzur-ykgM   559.29 ± 28.14  
  1. Notes: The promoter DNA regions upstream znuC, znuA and ykgM were cloned into the pRS551 plasmid, respectively, to fuse with the promoterless lacZ gene. β-Galactosidase activity (miller units) was detected to represent the promoter activity. Copy number of recombinant pRS551 was determined by real-time quantitative PCR, with the primers specific for the borne lacA gene. The detecting fold change of plasmid copy number was set to be 1 to generate a normalization factor that was subsequently used for generating the normalized fold change of promoter activity (miller units) in WT in relative to Δzur.