Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes

BMC Microbiology

Table 1 Summary of physiological and genetic data obtained for the Thiomonas strains used in this study.

Thiomonas strains	Arsenic-related phenotype/genotype								Growth with different electron donors^c					Influence of As(III) on final cell concentration^g
	As(III) Oxidation	MIC (mM)		Motility^a	Effect of arsenite on strain motility^b	PCR amplification of arsenic-related genes			As(III)^{d, e}	YE + As(III)^f	YE^f	YE + S₂O₃^2-	S₂O₃^2-e
		As(III)	As(V)			aoxAB	arsB1	arsB2						YE 0.1 g L^-1	YE 0.2 g L^-1
3As	+	10	100	2.9	/	+	+	+	-	+	++	+++	-	- (69%)	- (67%)
Ynys1	-	5	12.5	5.6	- (35%)	-	+	-	-	nd	-	+++	-	nd	nd
WJ68	+	10	> 100	38.7	+ (6%)	+	+	-	-	nd	++	+++	-	nd	nd
Tm. arsenivorans	+	10	100	4.5	+ (24%)	+	-	+	++	++	++	+++	++	+ (25%)	/
Tm. perometabolis	-	5	> 100	0	/	-	+	-	-	nd	-	+++	-	nd	nd

^a Diameter (mm) of swimming ring formed on 0.3% agar plates after 72 h incubation expressed as a difference with non motile strains (forming colonies of < 3 mm diameter); ^bMotility was tested in the presence of 1.33 mM of arsenite: "+" indicates a diameter of swimming ring greater than in absence of arsenite, "-" a smaller one and "/" no change. ^cBasel medium (MCSM or m126) amended with either yeast extract (YE), thiosulfate or arsenite or combinations thereof. ^d5,33 mM in case of 3As, WJ68, and Tm. arsenivorans, 2.67 mM in case of Ynys1 and Tm. perometabolis. ^eGrowth is expressed as an increase of colony forming units (cfu) observed after 10 days; -, no increase; ^fTested with 0.1, 0.2, 0.3% or 0.5% YE in absence of As(III), with 0.1, 0.2 or 0.3% YE and 1.3 mM of As(III), or with 0.3% YE and 2.6 mM As(III), except for WJ68, tested in 0.5% YE, without As(III). ^g1.33 mM As(III) in MCSM. nd: no data.

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